Cullen has been a key figure at the club, serving as an executive director and advisor to Levy for many years.
She has also been the Trustee of the Tottenham Hotspur Foundation, which supports various community initiatives and charitable programs in the UK and around the world.
In a statement, Cullen expressed her gratitude to the club and its staff, saying it has been a privilege to work with Levy and many other talented colleagues over the past three decades.
Cullen reflected on her time at the club, from her early days at White Hart Lane to the current stadium, and thanked her family for their support throughout her career.
She stated that she will spend the coming months ensuring a smooth transition of her responsibilities and wished the club continued success in the future.